Unit Two: Marine Microbiology - The Sea You Can't See

Activity 1: “The Small (Big) Picture”

Objective
Students will understand that despite their diminutive size, the high abundance of microbes (and in particular, bacteria) in the marine environment make them a significant factor in the cycling of matter and energy.

Background | Materials | Procedure | Discussion

Background
Bigger is better, right? Well, if we’re talking about desserts and bank accounts many people would probably agree with that statement. But what about things that are small – really small? Are they ever important or valuable or interesting? If you’re talking about living organisms on our planet then the answer is yes. And if you’re talking more specifically about life in the ocean, then the answer is definitely YES.

What comes to your mind when you think of life in the ocean? Many people immediately think of whales, dolphins, sharks, sea turtles, seals, sea otters or other animals. Sure, they’re cute and/or cool, but they have something else in common - they’re all macroorganisms, which means they’re big and visible. But what about the microorganisms – all the living things so small you can’t see them with your normal vision? What about life in the sea you can’t see?

Microbes are a diverse collection of single-celled organisms that have been on Earth for billions of years. They include bacteria, archaea (bacteria look-alikes), protists, fungi and viruses. Many people associate all microbes with germs and disease (and some do cause illness), but many microbes are very beneficial and even essential to other organisms. In fact, without microbes there wouldn’t be any other life on the planet, including us! For example, microbes in the ocean help produce the air we breathe, microbes in the soil help produce the food we eat, and microbes in our digestive system help process the food that we eat and make vitamins for us.

Microbes have evolved to live just about everywhere on the planet, and the ocean is no exception. From shallow bays and coral reefs to frozen polar waters and hydrothermal vents miles below the surface – microbes play a big role in how matter and energy move between organisms and their environment in the ocean.

Back to top

Materials
Computers with Internet access for groups of 3-4 students
For each group of students:

Calculator
1 lb. bag of rice
Measuring spoon
Cup
Bowl (large enough to hold all the rice from the bag)

Copies of the student worksheets for each student

Back to top

Procedure

  1. Divide students into groups of 3-4 based on the available number of computers, or preferred group size
  2. Ask students to briefly discuss in their groups what comes to mind when they are asked to think about microbes – review some of their answers as a class, and guide the discussion to focus on their size, abundance, and potential roles in the environment.
  3. Have the students review the Background information and then complete the following activities using the materials listed above:
    The Mission is Fission
    An Educated Guess
    Crunching the Numbers

The Mission is Fission
How can organisms as small as microbes play a big role in anything, you ask? Numbers …big numbers! Microbes may not be big individually, but they make up for their small size with their large numbers.

Click here to learn how microbes can be so abundant.


An Educated Guess
If you look around your classroom it probably isn’t too difficult to get an exact count or a close estimate of the number of people present. But what if that same room was filled with grains of rice – could you do the same? Assuming you didn’t have the time to count every grain, can you think of how you could get a good estimate of how many grains are in the room? Record any ideas on your worksheet.

Let’s make it a little easier and use a bag of rice instead …

Follow these steps in your group:

  1. Pass the bag of rice around the group and have each person estimate how many grains they think are in the bag and record their estimates on their worksheets
  2. Fill the measuring spoon with rice and count the number of grains – record the number on your worksheet
  3. Using the measuring spoon, fill the cup with rice - record the number of spoonfuls needed to fill the cup on your worksheet
  4. Calculate the number of grains in the cup - record the number on your worksheet
  5. Empty the cup of rice into the bowl
  6. Continue to fill and refill the cup to the same level, emptying the rice into the bowl each time, until the bag of rice is empty - record how many times you fill the cup, including the first time you did it to calculate how many grains are in a cup
  7. Calculate how many grains of rice were in the bag – record the number on your worksheet
  8. Compare your results with other groups

Scientists face the same dilemma when trying to imagine and measure the numbers of microbes in the ocean. They can’t count all the microbes directly, so instead they use extrapolation (x-trap-oh-lay-shun), which means they make estimates based on information they have already obtained. Scientists can take smaller samples at certain places and times in the ocean, count the microbes, and then use that information to make better estimates for larger areas. You just used extrapolation to determine how many grains of rice were in the bag assigned to your group. How much of a difference was there between your first estimate of the number of grains of rice in the bag compared to the number the group found using extrapolation?


Crunching the Numbers
Whether they are studying the speed of light or the number of bacteria in seawater, it can quickly become a burden for scientists to have to express very large or small numbers in the normal way known as standard notation. In response to this, scientists have developed a method known as scientific notation to make it easier to write and manipulate (multiplying, dividing) these long numbers.

All numbers can be expressed in scientific notation using a combination of three types of numbers:

  • A coefficient (greater than or equal to 1, but less than 10)
  • The base number 10 (which is always 10)
  • An exponent (a positive or negative number that indicates the power of the base number 10)

The general format for scientific notation can be expressed as N x 10x, where:
N = the coefficient
10 = the base
x = the exponent

To write a number in scientific notation:

  • Place a decimal after the first number
  • Count the number of places from the decimal to the end of the original number – then drop any zeros from the end of the number
  • The exponent is equal to the number of decimal places you counted

For example, let’s say you wanted to convert the large number 4,560,000,000 from standard notation into scientific notation. You would first place a decimal after the first number (4.560000000), then count the number of places from the decimal to the end of the number (in this example 9 places) to find your exponent for the base number 10. If you drop the zeros from the end of the number to create your coefficient you would get 4.56 x 109.

You can use the same method to express very small numbers. The only difference is that the exponent will be negative for numbers less than 1. For example, the number 0.000015, which is less than 1, would be expressed as 1.5 x 10-5.

Click here to practice converting numbers to and from scientific notation. Continue to do problems until you get at least five correct, and record your answers on your worksheet.


Questions for Discussion

  1. How many bacteria might be found in the ocean based on your previous calculation of how many bacteria are in a gallon of seawater and the following information? Be sure to use scientific notation for your answer.

    The ocean contains 3.28 x 108 cubic miles of water
    1 cubic mile = (5280)3 cubic feet of water
    1 cubic foot of water = (12)3 cubic inches of water
    1 gallon = 231 cubic inches of water
  2. Based on your knowledge of how many bacteria are in an average milliliter of seawater, how many might you get in a mouthful of seawater while swimming or bodysurfing at the beach?
  3. The very large numbers of microbes in the ocean lead us to believe they must be up to something. How do you think these microbes play a role how matter and energy moves between organisms in the ocean?
  4. If there are so many microbes in the ocean, and they can reproduce very quickly when conditions are right, why isn’t the ocean completely full of microbes? What factors can you think of that could control the numbers of microbes in the ocean?